MIA antibody (Internal Region)

Catalog No. ABIN5539869

The Goat Polyclonal anti-MIA antibody has been validated for ELISA, IHC and WB. It is suitable to detect MIA in samples from Human.

Quick Overview for MIA antibody (Internal Region) (ABIN5539869)

Target: MIA (Melanoma Inhibitory Activity (MIA))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MIA antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Grade: Verified

Product Details anti-MIA Antibody

Binding Specificity: Internal Region

Purpose: MIA / CD-RAP

Sequence: PKLADRKLCA DQE

Specificity: The immunizing peptide represents the N terminus of the mature protein. Reported variants represent identical protein: NP_006524.1, NP_001189482.1,

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen and Skeletal Muscle. Recommended concentration: 5 μg/mL.

Western Blot: Approx 14 kDa band observed in Human Glioma lysates (calculated MW of 14.5 kDa according to NP_006524.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MIA

Target: MIA (Melanoma Inhibitory Activity (MIA))

Alternative Name: MIA

Background: MIA, melanoma inhibitory activity, CD-RAP, melanoma-derived growth regulatory protein

Gene ID: 8190

NCBI Accession: NP_006524