NAT8L antibody (AA 211-223)

Catalog No. ABIN5539871

This Goat Polyclonal antibody specifically detects NAT8L in WB, ELISA, IF and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for NAT8L antibody (AA 211-223) (ABIN5539871)

Target: NAT8L (N-Acetyltransferase 8-Like (GCN5-Related, Putative) (NAT8L))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This NAT8L antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-NAT8L Antibody

Binding Specificity: AA 211-223

Purpose: Nat8l (mouse aa211-223)

Sequence: SVDSRFRGKG IAK

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 48-50 kDa band observed in fetal and adult Mouse, and in Human (Cerebellum) Brain lysates (calculated MW of 32.7 kDa according to Mouse NP_001001985.3 and 32.8 kDa according to Human NP_848652.2). The observed band was completely block

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HEK293 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NAT8L

Target: NAT8L (N-Acetyltransferase 8-Like (GCN5-Related, Putative) (NAT8L))

Alternative Name: Nat8l

Background: Nat8l, N-acetyltransferase 8-like, 1110038O08Rik, Shati, N-acetylaspartate synthetase, N-acetyltransferase 8-like protein, NAA synthetase, protein Shati

Gene ID: 339983, 269642, 289727

NCBI Accession: NP_001001985