NELL2 antibody (Internal Region)

Catalog No. ABIN5539873

This anti-NELL2 antibody is a Goat Polyclonal antibody detecting NELL2 in ELISA and IHC. Suitable for Human.

Quick Overview for NELL2 antibody (Internal Region) (ABIN5539873)

Target: NELL2 (NEL-Like 2 (Chicken) (NELL2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NELL2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-NELL2 Antibody

Binding Specificity: Internal Region

Purpose: NELL2

Sequence: DPCQADTIRN DITKT

Specificity: This antibody is expected to recognize all reported isoforms (NP_001138579.1, NP_006150.1, NP_001138581.1, NP_001138582.1). Reported variants represent identical protein: NP_001138580.1, NP_006150.1.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 5 μg/mL.

Western Blot: Preliminary experiments gave an approx 80 kDa band in Human Brain (cortex and hippocampus) lysates after 0.5 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NELL2

Target: NELL2 (NEL-Like 2 (Chicken) (NELL2))

Alternative Name: NELL2

Background: NELL2, NEL-like 2 (chicken), NRP2, NEL-like protein 2, NEL-related protein 2, neural epidermal growth factor-like 2, protein kinase C-binding protein NELL2

Gene ID: 4753, 54003, 81734

NCBI Accession: NP_001138579, NP_006150, NP_001138581, NP_001138582