VPS16 antibody (Internal Region)

Catalog No. ABIN5539891

The Goat Polyclonal anti-VPS16 antibody (ABIN5539891) specifically detects VPS16 in WB, ELISA and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for VPS16 antibody (Internal Region) (ABIN5539891)

Target: VPS16 (Vacuolar Protein Sorting-Associated Protein 16 Homolog (VPS16))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This VPS16 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-VPS16 Antibody

Binding Specificity: Internal Region

Purpose: VPS16 (isoform 1)

Sequence: KESQKADEYL REIQ

Specificity: This antibody is expected to recognize reported isoform 1 (NP_072097.2) only.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Uterus. Recommended concentration: 5 μg/mL.

Western Blot: Approx 100 kDa band observed in lysates of cell lines HepG2, HeLa and Jurkat and in lysates of Mouse Spleen and Rat Thymus (calculated MW of 94.7 kDa according to NP_072097.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for VPS16

Target: VPS16 (Vacuolar Protein Sorting-Associated Protein 16 Homolog (VPS16))

Alternative Name: VPS16

Background: VPS16, vacuolar protein sorting 16 homolog (S. cerevisiae), hVPS16, OTTHUMP00000030047, OTTHUMP00000030048, vacuolar protein sorting protein 16, vacuolar protein sorting-associated protein 16 homolog

Gene ID: 64601, 80743, 296159

NCBI Accession: NP_072097