ZIC3 antibody (AA 178-189)

Catalog No. ABIN5539913

This Goat Polyclonal antibody specifically detects ZIC3 in ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for ZIC3 antibody (AA 178-189) (ABIN5539913)

Target: ZIC3 (Zic Family Member 3 (ZIC3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ZIC3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-ZIC3 Antibody

Binding Specificity: AA 178-189

Purpose: ZIC3 (aa178-189)

Sequence: HVDNNQVHLG LR

Specificity: This antibody is expected to NOT cross-react with other ZIC proteins.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Prostate shows nuclear staining in select secretory cells. Recommended concentration, 5-10 μg/mL.

Western Blot: Preliminary experiments gave an approx 70 kDa band in Human, Mouse and Rat Brain lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ZIC3

Target: ZIC3 (Zic Family Member 3 (ZIC3))

Alternative Name: ZIC3

Background: ZIC3, Zic family member 3 (odd-paired homolog, Drosophila), HTX, HTX1, ZNF203, heterotaxy 1, zinc finger protein ZIC 3, zinc finger protein of the cerebellum 3

Gene ID: 7547, 22773, 367944

NCBI Accession: NP_003404