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Hsp31p antibody (AA 50-64)

This Goat Polyclonal antibody specifically detects Hsp31p in WB and ELISA. It exhibits reactivity toward Saccharomyces cerevisiae.
Catalog No. ABIN5539931

Quick Overview for Hsp31p antibody (AA 50-64) (ABIN5539931)

Target

Hsp31p (HSP31)

Reactivity

Saccharomyces cerevisiae

Host

Goat

Clonality

Polyclonal

Application

Western Blotting (WB), ELISA

Grade

Verified
  • Binding Specificity

    AA 50-64

    Purpose

    Hsp31p (yeast, aa50-64)

    Sequence

    ETGKFGWDEH SLAKD

    Cross-Reactivity

    Saccharomyces cerevisiae

    Purification

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    Isotype

    IgG
  • Application Notes

    Western Blot: Approx 24 kDa band observed in lysates of postdiauxic (overnight grown yeast cells, and no signal in KO grown under identical conditions. Cells grown in logarithmic phase show diminished signals as expected. (calculated MW of 25.7 kDa accordi

    Peptide ELISA: antibody detection limit dilution 1:128000.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 mg/mL

    Buffer

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Minimize freezing and thawing.

    Storage

    -20 °C

    Storage Comment

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target

    Hsp31p (HSP31)

    Alternative Name

    Hsp31p

    Background

    Hsp31p, YDR533C

    NCBI Accession

    NP_010822
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