Hsp31p antibody (AA 50-64)
Quick Overview for Hsp31p antibody (AA 50-64) (ABIN5539931)
Target
Reactivity
Host
Clonality
Application
Grade
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Binding Specificity
- AA 50-64
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Purpose
- Hsp31p (yeast, aa50-64)
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Sequence
- ETGKFGWDEH SLAKD
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Cross-Reactivity
- Saccharomyces cerevisiae
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Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Isotype
- IgG
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Application Notes
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Western Blot: Approx 24 kDa band observed in lysates of postdiauxic (overnight grown yeast cells, and no signal in KO grown under identical conditions. Cells grown in logarithmic phase show diminished signals as expected. (calculated MW of 25.7 kDa accordi
Peptide ELISA: antibody detection limit dilution 1:128000.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Minimize freezing and thawing.
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Storage
- -20 °C
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Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Hsp31p (HSP31)
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Alternative Name
- Hsp31p
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Background
- Hsp31p, YDR533C
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NCBI Accession
- NP_010822
Target
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