Intestinal Alkaline Phosphatase antibody (Biotin)

Catalog No. ABIN5539993

This anti-Intestinal Alkaline Phosphatase antibody is a Rabbit Polyclonal antibody detecting Intestinal Alkaline Phosphatase in WB and EIA. Suitable for Cow.

Quick Overview for Intestinal Alkaline Phosphatase antibody (Biotin) (ABIN5539993)

Target: Intestinal Alkaline Phosphatase (ALPI)

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Intestinal Alkaline Phosphatase antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Intestinal Alkaline Phosphatase Antibody (Biotin)

Specificity: This product detects bovine Alkaline phosphatase. Cross reactivity against Alkaline phosphatase from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-biotin, anti-rabbit serum as well as purified and partially purified Alkaline phosphatase [calf intestine].

Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer

Immunogen: Alkaline phosphatase from calf intestine

Application Details

Application Notes: Western Blot : 1/500-1/2,000. ELISA : 1/4,000-1/20,000. This antibody has been assayed against 1.0 μg of Alkaline phosphatase in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested.

Restrictions: For Research Use only

Handling

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Intestinal Alkaline Phosphatase

Target: Intestinal Alkaline Phosphatase (ALPI)

Alternative Name: alkaline phosphatase,alpi,iap

UniProt: P19111