Intestinal Alkaline Phosphatase antibody (HRP)

Catalog No. ABIN5539994

This Rabbit Polyclonal antibody specifically detects Intestinal Alkaline Phosphatase in WB and EIA. It exhibits reactivity toward Cow.

Quick Overview for Intestinal Alkaline Phosphatase antibody (HRP) (ABIN5539994)

Target: Intestinal Alkaline Phosphatase (ALPI)

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Intestinal Alkaline Phosphatase antibody is conjugated to HRP

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Intestinal Alkaline Phosphatase Antibody (HRP)

Specificity: This product detects bovine Alkaline phosphatase. Cross reactivity against Alkaline phosphatase from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Alkaline phosphatase [calf intestine].

Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer

Immunogen: Alkaline phosphatase from calf intestine

Application Details

Application Notes: Western Blot : 1/200-1/1,000. ELISA : 1/2,000-1/10,000. Anti Alkaline phosphatase has been assayed against 1.0 μg of Alkaline phosphatase [E.coli] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/3,500 to 1/15,000 of the reconstitution concentration is suggested.

Restrictions: For Research Use only

Handling

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Intestinal Alkaline Phosphatase

Target: Intestinal Alkaline Phosphatase (ALPI)

Alternative Name: alkaline phosphatase,alpi,iap

UniProt: P19111