Integrin beta 4 antibody

Catalog No. ABIN5540088

The Mouse Monoclonal anti-Integrin beta 4 antibody has been validated for WB, FACS, IP, IF, IHC (fro), ICC and EIA. It is suitable to detect Integrin beta 4 in samples from Human.

Quick Overview for Integrin beta 4 antibody (ABIN5540088)

Target: Integrin beta 4 (ITGB4)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin beta 4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Enzyme Immunoassay (EIA)

Clone: 58XB4

Product Details anti-Integrin beta 4 Antibody

Specificity: Binds specifically to integrin β4 chain(CD104) or β4 integrin, that associates with integrin α6 (CD49f) forms α6/β4 (CD49f/CD104) heterodimer. CD104 is expressed on epithelial cells (especially on the proliferative basal layer epithelial cells in skin), endothelial cells, Schwann cells, certain tumor cells and a subset of pre-T cells.

Purification: Purified

Immunogen: Immunization of Balb/c mice with Integrin beta4. The immunogen used to raise this antibody was the full length CD104 protein.

Isotype: IgG2a

Application Details

Application Notes: ELISA. Western blotting. Immunofluorescence. Flow Cytometry. Immunoprecipitation. Imunocytochemistry of paraformaldehyde fixed cells. Immunohistochemistry on Frozen Sections. See Reference 1 for Protocols.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Buffer System: PBS Preservatives: 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles. Shelf life: One year from despatch.

Target Details for Integrin beta 4

Target: Integrin beta 4 (ITGB4)

Alternative Name: cd104,integrin beta-4

Background: Integrins are heterodimers composed of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. Integrin b4 (CD104) associates with integrin α6 (CD49f) forming the α6/β4 (CD49f/CD104) heterodimer. CD104 is expressed on epithelial cells (especially on the proliferative basal layer epithelial cells in skin), endothelial cells, Schwann cells, certain tumor cells and a subset of pre-T cells. CD49f/CD104 is an adhesion receptor for laminins (especially laminin 5) and keratin filaments and is involved in the regulation of hemidesmosome formation and of cell proliferation and activation. CD104 is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

UniProt: P16144

Pathways: Integrin Complex