PRNP antibody

Catalog No. ABIN5540135

This Chicken Polyclonal antibody specifically detects PRNP in WB, ICC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PRNP antibody (ABIN5540135)

Target: PRNP (Prion Protein (PRNP))

Reactivity: Human, Mouse, Rat

Host: Chicken

Clonality: Polyclonal

Conjugate: This PRNP antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-PRNP Antibody

Purification: Purified

Immunogen: Chickens were immunized with a recombinant fragment of the human prion protein emulsified with Freund's complete and incomplete adjuvants. After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks. Affinity-purified antibodies were then prepared using an agarose column to which the recombinant protein fragments were covalently attached.Finally, affinity purified antibodies (50 ug/ml final concentration) were mixed with the IgY fraction (10 mg/ml final concentration) in a mixture containing glycerol (50% v/v) (to prevent freezing at -20°C) and 0.02% Sodium Azide.

Isotype: Ig Fraction

Application Details

Application Notes: Western Blot: 1/2500. Immunohistochemistry: 1/1000-1/5000. Quality Control: Antibodies were analyzed by Immunocytochemistry (1/1000 dilution) using cells transfected with the prion protein cDNA.

Restrictions: For Research Use only

Handling

Buffer: Buffer System: 0.9 % (w/v) Sodium Phosphate (10 mM, pH 7.2) buffered isotonic saline, 50 % (v/v) Glycerol, with 0.02 % (w/v) Sodium Azide as an anti-microbial agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Upon receipt, store undiluted (in aliquots) at -20°C. This products is photosensitive and should be protected from light. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Target Details for PRNP

Target: PRNP (Prion Protein (PRNP))

Alternative Name: cd230,prp

Background: Prion protein is the protein responsible for human Creutzfeldt-Jakob Disease (CJD), and somatic mutations of the prion gene are responsible for Gerstmann-Straussler-Scheinker disease and Fatal Familial Insomnia. The bovine homolog of the prion protein is responsible for bovine Spongioform Encephalopathy (BSE), also known as Mad Cow Disease. In all cases, the prion protein has two folding states, with one state being the normal non-pathological form, and the other state inducing a spongioform encephalopathy.

UniProt: P23907

Pathways: Transition Metal Ion Homeostasis, Activated T Cell Proliferation