CD51 antibody

Catalog No. ABIN5540182

This anti-CD51 antibody is a Mouse Monoclonal antibody detecting CD51 in FACS, IP, IHC (fro) and EIA. Suitable for Human.

Quick Overview for CD51 antibody (ABIN5540182)

Target: CD51 (ITGAV) (Integrin alpha V (ITGAV))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD51 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: NKI-M7 (former AMF7)

Product Details anti-CD51 Antibody

Specificity: T-lymphocyte clones NKI-M7 detect a melanoma-associated proteoglycan (MW greater than 450-250 kDa) and a molecular complex, which under reducing conditions consists of 4 compounds of 120, 95, 29 and 25 kDa respectively. reacts with cultured melanomas melanomas in frozen sections. The antigen detected by is strongly associated with the adhesion and cytoplasmic spreading of melanoma cells to plastic surfaces and monolayers of vascular endothelial cells. blocks Fibronectin-induced chemotaxic motility and chemokinesis of melanoma cells. In addition to their membrane localization, the antigens detected by NKI-M7 are abundant in extracellular adhesion plaques deposited by cultured melanoma cells. stains primary tumors heterogeneously, whereas metastases are homogeneously stained.

Purification: Purified

Immunogen: Derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with cultured melanoma cells derived from a Human melanoma metastasis.

Isotype: IgG1

Application Details

Application Notes: ELISA. Fow Cytometry. Immunoprecipitation. Immunohistochemistry on Frozen Sections.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Buffer System: PBS Preservatives: 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD51

Target: CD51 (ITGAV) (Integrin alpha V (ITGAV))

Alternative Name: cd51,itgav

Background: CD51 (Cluster of Differentiation 51), known as vitronectin receptor α chain, or integrin αV is a type I integral membrane glycoprotein. CD51 forms a heterodimer with integrin β1 (CD29), β3 (CD61), β5, β6, or β8. CD51 contains two disulfide-linked subunits of 125 kD and 24 kD. Many extracellular matrix proteins with RGD-motifs are CD51 ligands. In association with its β chains, CD51 binds vitronectin, von Willebrand factor, fibronectin, thrombospondin, osteopontin, fibrinogen, and laminin. CD51, as an adhesion molecule, plays important roles in leukocytes homing and rolling, mediates bone absorption and angiogenesis. CD51 is expressed on endothelial cells, fibroblasts, macrophages, platelets, osteoclasts, neuroblastoma, melanoma, and hepatoma cells.

UniProt: P06756

Pathways: Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Integrin Complex