CD59 antibody

Catalog No. ABIN5540192

This Mouse Monoclonal antibody specifically detects CD59 in WB, FACS, Func and IHC (fro). It exhibits reactivity toward Rat.

Quick Overview for CD59 antibody (ABIN5540192)

Target: CD59

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD59 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: TH9

Product Details anti-CD59 Antibody

Specificity: The monoclonal antibody TH9 recognizes Rat CD59. Other species not tested.

Purification: Purified

Immunogen: Partially purified (FPLC) Rat CD59 prep (RIP)

Isotype: IgG1

Application Details

Application Notes: Western blot. Functional Studies. Flow Cytometry. Immunohistochemistry on Frozen Sections. The typical starting working dilution is 1/50.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Buffer System: PBS Stabilizers: 0.1 % BSA

Storage: 4 °C

Storage Comment: Store undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD59

Target: CD59

Alternative Name: cd59

Background: Rat CD59 was previously known as rat inhibitory protein (RIP) and it is a potent inhibitor of the complement membrane attack complex (MAC) action. CD59 regulates the formation and function of the lytic C5b-9 complex by binding C8 and preventing the unfolding and membrane insertion of C9 and by binding C9 and restricting its polymerization. CD59 is a small (18 - 25 kDa) molecule, linked to the cell membrane through a glycosyl phosphatidylinositol (GPI) anchor and comprising 77 amino acids with a single N-linked carbohydrate group at Asn-18. Analogues of CD59 can be found in all species with similar structures and sizes. In rat, CD59 is expressed on vacular endothelium and circulating cells. In the central nervous system (CNS) of the rat, CD59 is expressed on the Schwann sheath of peripheral nerve fibres and on ependymal cells, but not on glial cells and neurons in the CNS. Rat astrocytes in vitro express CD59 on its surface. CD59 may be involved in rheumatoid arthritis, motor nerve injury in the Guillain-Barré sydrome and in other diseases where defective inhibition of complement activation on self tissue is involved. Furthermore, CD59 may play an important part in abrogating the effects of complement attack in renal disease. Its presence and protective effect have already been demonstrated on human renal cells.

UniProt: P27274

Pathways: Complement System