Cytokeratin 1 antibody (pan) (FITC)

Catalog No. ABIN5540281

This anti-Cytokeratin 1 antibody is a Mouse Monoclonal antibody detecting Cytokeratin 1 in FACS, ICC and IF. Suitable for Human, Rat, Mouse, Cow, Rabbit, Chicken, Monkey and Pig.

Quick Overview for Cytokeratin 1 antibody (pan) (FITC) (ABIN5540281)

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Reactivity: Human, Rat, Mouse, Cow, Rabbit, Chicken, Monkey, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 1 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: AE1-AE3

Product Details anti-Cytokeratin 1 Antibody (FITC)

Binding Specificity: pan

Specificity: AE 1/AE3 represents an excellent marker for distinguishing carcinomas from non-epithelial tumors reacts with all epithelium-derived tumors and their neoplasms. Decorates the majority of type I and type II keratins (formerly also designated cytokeratins). This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas. This antibody AE 1/AE 3 recognizes Low Molecular Weight Cytokeratins (CK 10 (56.5), CK14 (50), CK15 (50), CK16 (48) and CK19 (40) of the acidic family and CK1 (67), CK2 (65.5), CK3 (64), CK4 (59), CK5 (58) CK6 (56) and CK8 (52.5).

Purification: Protein A Chromatography

Immunogen: Human epidermal keratin.

Isotype: IgG1

Application Details

Application Notes: Immunofluorescence: 10-20 μg /mL (1/10-1/20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4 °C. Flow Cytometry: 0.2-1.0 μg/0.1 mL (1/200-1/1,000) (Not tested in our lab).

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Target Details for Cytokeratin 1

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Alternative Name: cytokeratin

Background: Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1(67), CK2(65.5), CK3(64), CK4(59), CK5(58), CK6(56), CK7(54), CK8(52.5) and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10 (56.5), CK12 (56), CK13 (53), CK14 (50), CK16 (48), CK17 (46), CK18 (45), CK19 (48) and CK20 (46).