Endomucin antibody (AA 20-190)

Catalog No. ABIN5540322

The Rabbit Polyclonal anti-Endomucin antibody has been validated for WB and FACS. It is suitable to detect Endomucin in samples from Mouse.

Quick Overview for Endomucin antibody (AA 20-190) (ABIN5540322)

Target: Endomucin (EMCN)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Endomucin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-Endomucin Antibody

Binding Specificity: AA 20-190

Specificity: This antibody detects Mouse Endomucin (Glu20-Ser190). Other species not tested.

Purification: Protein A Chromatography

Immunogen: Highly pure (> 95%) recombinant Mouse soluble Endomucin (Glu20-Ser190) derived from E. coli (Cat.-No. AR60010PU-N)

Isotype: IgG

Application Details

Application Notes: Western blot: 1-5 μg/mL. FACS: 1-5 μg/mL.

Restrictions: For Research Use only

Handling

Buffer: PBS

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 month or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Endomucin

Target: Endomucin (EMCN)

Alternative Name: endomucin

Background: Endomucin (endothelial sialomucin also Endomucin-1/2 and Mucin-14) is an 80 - 120 kDa glycoprotein member of the Endomucin family of proteins. It is expressed on endothelial cells and depending upon its glycosylation pattern, can serve as either a pro- or anti-adhesive molecule. Mouse Endomucin precursor is 261 amino acids in length. It is type I transmembrane protein that contains a 170 aa extracellular domain (ECD) (aa 21 - 190) and a 50 aa cytoplasmic region. Three splice variants exist in the ECD. One shows a deletion of aa 91 - 141, a second shows a one aa substitution for aa 91 - 129, and a third shows a one aa substitution for aa 129 - 142. Over aa 21 - 90, mouse Endomucin shares 60 % and 30 % aa identity with rat and human Endomucin, respectively.