IL-10 antibody

Catalog No. ABIN5540435

This Mouse Monoclonal antibody specifically detects IL-10 in WB, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for IL-10 antibody (ABIN5540435)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-10 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-IL-10 Antibody

Specificity: This antibody detects Human Interleukin 10. Other species not tested.

Purification: Affinity Chromatography on Protein A

Immunogen: Highly pure (> 98%) E.coli derived Recombinant Human IL-10 (Cat.-No PA084).

Isotype: IgG1

Application Details

Application Notes: Sandwich ELISA (assuming 100 μL/well), a concentration of 8.0-10.0 μg/mL of this antibody will detect at least 1000 pg/well of recombinant Human IL-10 when used with Biotinylated Antigen Affinity purified anti-Human IL-10 (Cat.-No PP1032B) as the detection antibody at a concentration of approximately 0.05-0.1 μg/mL. Western Blot: To detect hIL-10 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions. Immunohistochemistry on Paraffin Sections: This antibody stained formalin-fixed paraffin-embedded sections of human pancreas adenocarcinoma tissue. The recommended concentration is 5.0 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.

Restrictions: For Research Use only

Handling

Buffer: PBS without preservatives

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: interleukin-10,il10

Background: Interleukin 10 (IL-10) is a 178 amino acid protein that is primarily secreted by TH2 clones. IL-10 has dual functions, the first of which is the suppression of cytokine production by TH1 clones responding to antigen presented by monocyte and macrophage antigen presenting cells (APCs). The second function consists of the inhibition of response of cytokine targeted cells, possibly by the downregulation of CD25 (the interleukin-2 receptor) on macrophages and B lymphocytes. IL-10 exerts its biological activity through the IL-10 receptor (IL-10R) a 560 amino acid, 110 kDa glycoprotein whose expression can be induced in cultured macrophages and fibroblasts by elevated in HIV-1 infected individuals and has been implicated in the progression of the disease.

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints