Layilin antibody (AA 22-235)

Catalog No. ABIN5540461

This anti-Layilin antibody is a Mouse Monoclonal antibody detecting Layilin in WB and EIA. Suitable for Human.

Quick Overview for Layilin antibody (AA 22-235) (ABIN5540461)

Target: Layilin (LAYN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Layilin antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: AT20G8

Product Details anti-Layilin Antibody

Binding Specificity: AA 22-235

Purification: Protein-A affinity chromatography

Immunogen: Recombinant human LAYN (22-235aa) purified from E. coli

Isotype: IgG2b

Application Details

Application Notes: The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:500.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4 containing 0.02 % Sodium Azide and 10 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Layilin

Target: Layilin (LAYN)

Alternative Name: layilin

Background: Layilin is a widely expressed integral membrane hyaluronan receptor and a transmembrane protein with homology to C-type lectins. This protein interacts with the ERM protein family of cytoskeletal membrane linker molecule members, including talin, ezrin, moesin, and radixin. The carboxy-terminal domain of layilin is capable of binding to the amino-terminal domain of radixin. An interdomain interaction between the amino- and the carboxy-terminal domains of radixin inhibits its ability to bind to layilin. Layilin plays an important role in cell adhesion and motility. It is important for the maintenance of the cortical structure in the cell because it mediates signals from the extracellular matrix to the cytoskeleton. In spreading cells, Layilin acts as a membrane-binding site for talin in the peripheral ruffles, and it may play a role in the migration of cells to a wound site.

UniProt: Q6UX15