s100a4 antibody

Catalog No. ABIN5540655

The Mouse Monoclonal anti-s100a4 antibody has been validated for FACS, EIA, ELISA (Capture) and ELISA (Detection). It is suitable to detect s100a4 in samples from Human.

Quick Overview for s100a4 antibody (ABIN5540655)

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This s100a4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Enzyme Immunoassay (EIA), ELISA (Capture), ELISA (Detection)

Clone: NJ-4F3

Product Details anti-s100a4 Antibody

Specificity: Recognizes Human S100 Calcium Binding Protein A4 (S100A4). Other species not tested.

Purification: Affinity Chromatography on Protein G

Immunogen: Genetic immunisation with cDNA encoding S100A4. Selection: based on recognition of the complete native protein expressed on transfected mammalian cells.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1.2 μg/10 6 cells. ELISA: 1/200-1/400. ELISA (capture): Clone NJ-4F3-D1 as detection antibody. ELISA (detection): Clone NJ-5C6-A3 as capture antibody. Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Phosphate buffered saline, pH 7.2

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for s100a4

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Alternative Name: s100a4

Background: S100 calcium binding protein A4 (S100A4) is a member of the S100 family of calcium-binding proteins that contain two Ca(2+)-binding sites including a canonical EF-hand motif. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells. S100A4 interacts with cytoskeletal proteins and enhances metastasis of several types of cancer cells. It is secreted by unknown mechanisms, thus, paracrinely stimulating a variety of cellular responses, including angiogenesis and neuronal growth (1). S100A4 has been shown to be a prognostic marker in a number of human cancers, including esophageal-squamous cancers, non-small lung cancers, primary gastric cancers, malignant melanomas, prostate cancers, bladder cancers, and pancreatic carcinomas. The universality of S100A4 expression in a variety of cancers illustrates the potential use of S100A4 as a marker for tumor metastasis and disease progression (2).

UniProt: P26447

Pathways: S100 Proteins