Small Basement Membrane Heparan Sulfate Proteoglycan antibody

Catalog No. ABIN5540680

The Mouse Monoclonal anti-Small Basement Membrane Heparan Sulfate Proteoglycan antibody (Clone 1F10-B8) (ABIN5540680) specifically detects Small Basement Membrane Heparan Sulfate Proteoglycan in WB, EIA and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for Small Basement Membrane Heparan Sulfate Proteoglycan antibody (ABIN5540680)

Target: Small Basement Membrane Heparan Sulfate Proteoglycan

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 1F10-B8

Product Details

Specificity: This Monoclonal antibody 1F10/B8 is specific for a core protein epitope of a Human small basement membrane Heparan Sulfate proteo-glycan (HS-PG). AM02102PU recognizes an epitope different from that recognized by clones 2H7/G11 (Cat.-No AM02103PU) and 2E2/B5 (Cat.-No AM20231PU). Species: Human.

Purification: Protein G Chromatography

Immunogen: Purified Human small basement membrane Heparan Sulfate Proteoglycan (HS-PG)

Isotype: IgG1

Application Details

Application Notes: ELISA (less than 1 μg/mL). Western blot (1 μg/mL). Immunohistochemistry (cryosections, 1 μg/mL).

Protocol: 1. Stöcker G, Meyer HE, Wagener C and Greiling H (1991): Purification and N-terminal amino acid sequence of a chondroitin sulfate / dermatan sulfate proteoglycan isolated from intima / media preparations of human aorta. Biochem J 274: 415-420. 2. Heintz B, Stöcker G, Rentz U, Melzer H, Mrowka C, Stickeler E, Sieberth HG, Greiling H, Haubeck HD (1995): Decreased glomerular basement membrane heparan sulfate proteoglycan in essential hypertension. Hypertension 25: 399-407. 3. Stefanidis I, Heintz B, Stöcker G, Mrowka C, Sieberth HG, Haubeck HD (1996): Association between heparan sulfate proteoglycan excretion and proteinuria after renal transplantation. J Am Soc Nephrol 7: 1-7. 4. Stöcker G, Stickeler E, Switalla S, Fischer DC, Greiling H, Haubeck HD (1997): Development of an enzyme immuno assay specific for a core protein epitope of a novel small basement membrane associated heparan sulphate proteoglycan from human kidney. Eur J Clin Chem Clin Biochem 35: 95-99.

Restrictions: For Research Use only

Handling

Buffer: 50 mM TRIS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details

Target: Small Basement Membrane Heparan Sulfate Proteoglycan

Background: The principal molecular structure of basement membranes has been elucidated during the past two decades and it was shown that for example in the glomerular basement membrane (GBM) HS-PG are responsible for the selective filtration process. Removal of HS-PG will lead to proteinuria. Whereas previously perlecan was the only known basement membrane HS-PG, there is now evidence that (at least two) other basement membrane HS-PG do exist: Agrin, originally discovered as an important component of the neuromuscular junction and a novel small HS-PG, that was isolated from human aorta and kidney. This HS-PG, with a molecular weight of 80-200 kDa (aorta) and 30-160 kDa (kidney) and a core protein size of 24 kDa or 22 kDa, respectively, was localized by immunohisto-chemistry to the basement membrane. Amino acid sequence analysis of tryptic peptides indicate, that this small HS-PG is clearly distinct from perlecan and agrin.

UniProt: P34741