alpha Fetoprotein antibody (FITC)

Catalog No. ABIN5540745

The Rabbit Polyclonal anti-alpha Fetoprotein antibody is suitable to detect alpha Fetoprotein in samples from Human. It has been validated for IF, ICC, EIA and IHC (fro).

Quick Overview for alpha Fetoprotein antibody (FITC) (ABIN5540745)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Fetoprotein antibody is conjugated to FITC

Application: Immunofluorescence (IF), Immunocytochemistry (ICC), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-alpha Fetoprotein Antibody (FITC)

Specificity: The reactivity of the antiserum is restricted to alpha-foetoprotein. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against amniotic fluid, hepatoma sera and purified alpha-foetoprotein a single precipitin line is obtained. No precipitation reaction is obtained with normal human serum. Cross-reactivity: The antiserum does not cross react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross reactivity is a normal feature of antibodies to mammalian serum proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail. Species: Human.

Immunogen: Purified Human Alpha-Foetoprotein is isolated from pooled amniotic fluid and hepatoma serum, using a proprietary multiple step method. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Can be used to identify and measure Alpha Foetoprotein at the cellular and subcellular level by Immunofluorescence staining of appropriately treated cell and tissue substrates, This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions : 1/20-1/80.

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Alternative Name: alpha-fetoprotein,afp

Background: Several foetoproteins have been demonstrated in human foetal serum and amniotic fluid. One of the best studied is the alphafoetoprotein. It cab easily be identified immunochemically because of its distinct antigenicity. In electrophoresis its relative mobility is between the albumin and alpha-1 globulin fractions. Its molecular weight is 64,000, the carbohydrate content is relatively low. The concentration of alpha-foetoprotein gradually decreases during foetal growth. It is not found in normal adult serum, but reappears in most human developing hepatoma. In pooled foetal

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process