Cytokeratin 5 antibody (AA 1-590)

Catalog No. ABIN5540901

The Mouse Monoclonal anti-Cytokeratin 5 antibody has been validated for WB and EIA. It is suitable to detect Cytokeratin 5 in samples from Human.

Quick Overview for Cytokeratin 5 antibody (AA 1-590) (ABIN5540901)

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 5 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: AT12C4

Product Details anti-Cytokeratin 5 Antibody

Binding Specificity: AA 1-590

Purification: Protein-A affinity chromatography

Immunogen: Recombinant human KRT5 (1-590aa) purified from E. coli.

Isotype: IgG2a

Application Details

Application Notes: The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid. In Phosphate-Buffered Saline ( pH 7.4) with 0.02 % Sodium Azide, 10 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Cytokeratin 5

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Alternative Name: cytokeratin 5

Background: Keratin 5, also known as KRT5, K5, or CK5, dimerizes with keratin 14 and forms the intermediate filaments(IF) that make up the cytoskeleton of basal epithelial cells. This protein is involved in several diseases including epidermolysis bullosa simplex and breast and lung cancers. Keratin 5(and K14) are expressed primarily in basal keratinocytes in the epidermis, specifically in the stratified epithelium lining the skin and digestive tract. Keratin intermediate filaments make up the cytoskeletal scaffold within epithelial cells, which contributes to the cell architecture and provides the cells with the ability to withstand mechanical, and non-mechanical, stresses. K5/K14 keratin pairs are able to undergo extensive bundling due to the non-helical tail of K15 acting as a weak cross-linker at the intermediate filament surface. This bundling increase the elasticity, and therefore the mechanical resilience, of the intermediate filaments.

UniProt: P13647