NEFH antibody

Catalog No. ABIN5540977

This anti-NEFH antibody is a Mouse Monoclonal antibody detecting NEFH in WB and IHC (fro). Suitable for Human, Rat, Mouse, Chicken, Cow, Rabbit, Guinea Pig, Dog, Sheep, Xenopus laevis, Hamster and Zebrafish (Danio rerio).

Quick Overview for NEFH antibody (ABIN5540977)

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivity: Human, Rat, Mouse, Chicken, Cow, Rabbit, Guinea Pig, Dog, Sheep, Xenopus laevis, Hamster, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEFH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: RNF405

Product Details anti-NEFH Antibody

Specificity: RNF405 reacts exclusively with the phosphorylated isoform of the 200 kD Neurofilament protein.

Purification: Purified

Immunogen: Neurofilament preparation of calf brain tissue.

Isotype: IgG2a

Application Details

Application Notes: Immunoblotting. Immunofluorescence. Immunohistochemistry on Frozen Sections. Recommended Dilutions : 1/50-1/100 for Immunohistochemistry with avidin biotinylated Horseradish Peroxidase complex (ABC) as detection reagent, and 1/100-1/500 for Immunoblotting applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Buffer System: PBS Preservatives: 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles. Shelf life: One year from despatch.

Target Details for NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Alternative Name: neurofilament h (200 kd)

Background: Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.

UniProt: P12036