ALDOA antibody (HRP)

Catalog No. ABIN5541023

This Goat Polyclonal antibody specifically detects ALDOA in WB, IP and EIA. It exhibits reactivity toward Human and Rabbit.

Quick Overview for ALDOA antibody (HRP) (ABIN5541023)

Target: ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))

Reactivity: Human, Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This ALDOA antibody is conjugated to HRP

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-ALDOA Antibody (HRP)

Specificity: This antibody detects rabbit aldolase. Cross reactivity against aldolase from human tissues is known. Crossactivity against aldolase from other tissues and species may also occur. Immunoelectrophoresis gives a single precipitin arc against anti-goat serum, purified and partially purified aldolase [Rabbit muscle]. It has been reported that this antibody can detect human Aldolase on immunoblot showing a 41 kDa band in lysates from MCF7, NMB231 and HBL100 cell lines. Species: Rabbit, Human.

Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer

Immunogen: Aldolase from rabbit muscle

Application Details

Application Notes: Western blot :1/500-1/5,000. Immunoprecipitation : 1/100. Use approximately 5 μL of antibody to immunoprecipitate 50 μL of protein lysate. ELISA : 1/5000-1/20,000. This antibody has been assayed against 1.0 μg of aldolase [rabbit muscle] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/2,000 of the reconstitution concentration is suggested.

Restrictions: For Research Use only

Handling

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for ALDOA

Target: ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))

Alternative Name: aldolase a,aldoa

UniProt: P00883

Pathways: Ribonucleoside Biosynthetic Process