CTLA4 antibody (AA 36-161)

Catalog No. ABIN5541119

This Mouse Monoclonal antibody specifically detects CTLA4 in FACS, WB, ICC, IF and EIA. It exhibits reactivity toward Human.

Quick Overview for CTLA4 antibody (AA 36-161) (ABIN5541119)

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTLA4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Clone: AT4F2

Product Details anti-CTLA4 Antibody

Binding Specificity: AA 36-161

Specificity: This antibody detects CTLA4 at aa 36-161.

Purification: Protein-A affinity chromatography

Immunogen: Recombinant human CTLA4 (36-161aa) purified from E. coli

Isotype: IgG1

Application Details

Application Notes: ELISA. Western blot: Recommended starting dilution is 1:3000. Immunocytochemistry / Immunoflourescence. Flow cytometry.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4 containing 0.02 % Sodium Azide and 10 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CTLA4

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Alternative Name: cd152,ctla4

Background: CTLA4 (Cytotoxic T-Lymphocyte Antigen 4), also known as CD152, is a protein receptor that downregulates the immune system. CTLA4 is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Mutations in CTLA4 have been associated with insulin-dependent diabetes mellitus, Graves' disease, Hashimoto's thyroiditis, celiac disease and other autoimmune diseases.

UniProt: P16410

Pathways: Cancer Immune Checkpoints