CEACAM5 antibody

Catalog No. ABIN5541181

This anti-CEACAM5 antibody is a Mouse Monoclonal antibody detecting CEACAM5 in FACS, IF, WB, IHC (fro), EIA and ICC. Suitable for Human.

Quick Overview for CEACAM5 antibody (ABIN5541181)

Target: CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CEACAM5 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunocytochemistry (ICC)

Clone: 26-5-1

Product Details anti-CEACAM5 Antibody

Specificity: This antibody reacts to CD66e / CEACAM5. Species: Human.

Purification: Affinity Chromatography on Protein G

Immunogen: Immunisation with extracted protein of CEACAM5

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: 1.2 μg/10 6 cells. ELISA: 1/200-1/400. Cell based ELISA with intakt, transiently transfected cells: 1/200. Western blot: 4 μg/mL. Immunohistochemistry on Frozen Sections: 1-2 μg/10 cells (on cryosections).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Phosphate buffered saline, pH 7.2

Storage: 4 °C,-20 °C

Storage Comment: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CEACAM5

Target: CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5))

Alternative Name: cd66e,ceacam5

Background: CEACAM5 (CEA-related cell adhesion molecule 5, CEA) belongs to the carcinoembryonic antigen (CEA) gene family (1,2). It encodes a glycosyl phosphatidyl inositol (GPI)-linked glycoprotein with a Mr of 180,000-200,000 which is most strongly expressed on epithelial cells of the fetal and adult colon and to a minor extent on epithelial cells of the stomach and sweat glands, squamous epithelial cell of the tongue, esophagus and cervix. CEACAM5 is used as a tumor marker for early detection of recurrent disease due to its expression in adenocarcinomas of the colon, lung, breast, stomach and pancreas and in mucinous ovarian carcinomas (3). Like all members of the CEACAM family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by six immunoglobulin constant-like A (A1, A2, A3) and B domains (B1, B2, B3).

UniProt: P06731