ESM1 antibody (AA 19-184)

Catalog No. ABIN5541435

The Rabbit Polyclonal anti-ESM1 antibody has been validated for WB. It is suitable to detect ESM1 in samples from Mouse.

Quick Overview for ESM1 antibody (AA 19-184) (ABIN5541435)

Target: ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ESM1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ESM1 Antibody

Binding Specificity: AA 19-184

Specificity: This antibody detects Mouse Endocan / ESM1. Other species not tested.

Purification: Protein A Chromatography

Immunogen: Highly pure (> 95%) recombinant human Endocan/ESM1 (Trp19-Arg184) derived from E. coli

Isotype: IgG

Application Details

Application Notes: Western blot: 1-5 μg/mL.

Restrictions: For Research Use only

Handling

Buffer: 5 mM PBS, pH 7.2

Storage: 4 °C,-20 °C

Storage Comment: Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for ESM1

Target: ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))

Alternative Name: esm1

Background: Endocan, also known as endothelial cell-specific molecule1 (ESM1), is a secreted cysteine-rich dermatan sulfate (DS) proteoglycan primarily expressed by endothelial cells within the vascular capillary network in kidney and in the alveolar walls of the lung. Endocan expression has also been detected in different epithelia and in adipocytes. The expression of endocan is up-regulated by TNFα, IL1β or lipopolysaccharide and down-regulated by IFNγ. The mouse Endocan gene encodes a 184 amino acid (aa) residues precursor protein with a 19 aa hydrophobic signal peptide and a 165 aa mature region with 18 Cysteine residues. The DS chain is covalently attached to serine 137. Endocan has been shown to bind CD11a/CD18 integrin (also known as lymphocyte function-associated antigen1, LFA1) on human lymphocytes, monocytes and Jurkat cells, inhibiting its binding to ICAM1 and reducing LFA1mediated leukocyte activation. Endocan binds via its DS chain to hepatocyte growth factor (HGF) to enhance HGF mitogenic activity. Genetically engineered cells overexpressing Endocan has been shown to induce tumor formation, suggesting that Endocan may be involved in the pathophysiology of tumor growth in vivo. Circulating Endocan can be detected in the serum from healthy subjects.

Pathways: Growth Factor Binding