beta-Galactosidase Tag antibody (Biotin)

Catalog No. ABIN5541541

This anti-beta-Galactosidase Tag antibody is a Rabbit Polyclonal antibody detecting beta-Galactosidase Tag in WB, EIA, IHC (p) and IHC (fro). Suitable for .

Quick Overview for beta-Galactosidase Tag antibody (Biotin) (ABIN5541541)

Target: beta-Galactosidase Tag

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta-Galactosidase Tag antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-beta-Galactosidase Tag Antibody (Biotin)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum as well as purified and partially purified Beta-galactosidase (E.coli).

Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer

Immunogen: Beta-galactosidase from E.coli

Application Details

Application Notes: Western blot : 1/2,000-1/10,000. ELISA : 1/10,000-1/50,000. IHC : 1/1,000-1/5,000. The antibody recognizes both frozen tissue sections, paraffin embedded tissue and 4 % paraformaldehyde fixed tissue for most immunohistochemical analysis : A 1:1,500 dilution has been reported to detect beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ. In this particular experiment, tissue was perfused with 4 % paraformaldehyde and cryostat-cut (35 μm) to produce free-floating sections. A 1:5,000 dilution has been reported to be successful for staining of brain sections from transgenic mice expressing nuclear beta-galactosidase when assayed by immunofluorescence microscopy. A 1:5,000 dilution has been reported for immunofluorescent staining of methanol fixed, devitellinized Drosophila embryos. A 1:5,000 dilution has been reported to be successful for staining by immunoblot of beta-galactosidase fusion proteins after transfer using a semi-dry transfer apparatus.

Restrictions: For Research Use only

Handling

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for beta-Galactosidase Tag

Target: beta-Galactosidase Tag

Target Type: Tag

UniProt: P00722