CD13 antibody (FITC)

Catalog No. ABIN5541585

The Rat Monoclonal anti-CD13 antibody has been validated for FACS. It is suitable to detect CD13 in samples from Mouse.

Quick Overview for CD13 antibody (FITC) (ABIN5541585)

Target: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD13 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: 123H1

Product Details anti-CD13 Antibody (FITC)

Specificity: This antibody reacts with mouse CD13.

Purification: Protein G agarose

Immunogen: Murine dendritic cells isolated from C57BL/6 mice

Isotype: IgG2b

Application Details

Application Notes: Flow cytometry: 10 μg/m (final concentration). For details see protocol below.

Protocol: Flow cytometric analysis for floating cells We usually use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2 % fetal calf serum (FCS) and 0.1 % NaN 3]. 2) Resuspend the cells with washing buffer (5x10 6 cells/mL). 3) Add 50 μ L of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute at room temperature (20~25 o C). Remove supernatant by careful aspiration. 4) Add 10 μ L of normal goat serum containing 1 mg/mL normal human IgG and 0.1 % NaN 3 to the cell pellet after tapping. Mix well and incubate for 5 minutes at room temperature. 5) Add 40 μ L of the primary antibody at the concentration of as suggest in the APPLICATIONS diluted in the washing buffer. Mix well and incubate for 30 minutes at room temperature. 6) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at room temperature. Remove supernatant by careful aspiration. 7) Resuspend the cells with 500 μ L of the washing buffer and analyze by a flow cytometer. (Positive controls for Flow cytometry JAWSII, C2C12)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.09 % NaN 3 Stabilizers: 1 % BSA

Storage: 4 °C

Storage Comment: Store at 2-8 °C. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD13

Target: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Alternative Name: cd13

Background: CD13 is a myeloid differentiation molecule expressed on committed myeloid progenitors, granulocytes, monocytes, and leukemic cells of myeloid origin. It is also expressed on non-hemopoietic cells including fibroblasts, renal proximal tubule, and small intestine brush-border membrane. The gene for CD13 has been cloned the cDNA sequence shows that CD13 is the metalloprotease aminopeptidase N. Biochemical studies have shown that CD13 is a 150 kDa glycoprotein, which exists as a 130 kDa intracellular precursor form. This 130 kDa precursor molecule is pos ttranslationally modified in the Golgi apparatus to produce the 150 kDa mature cell surface form of the molecule.

UniProt: P97449

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones