PARP1 antibody (AA 373-540)

Catalog No. ABIN5541684

The Mouse Monoclonal anti-PARP1 antibody has been validated for WB and IHC (p). It is suitable to detect PARP1 in samples from Human.

Quick Overview for PARP1 antibody (AA 373-540) (ABIN5541684)

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PARP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 839120

Product Details anti-PARP1 Antibody

Binding Specificity: AA 373-540

Specificity: The antibody recognizes Human PARP. Other species not tested.

Purification: Affinity Chromatography on Protein A/G

Immunogen: E.coli -derived recombinant human PARP- Thr 373-Glu 540

Isotype: IgG1

Application Details

Application Notes: Western Blot: 1 μg/mL. Positive Control : HeLa cell lysate. Immunohistochemistry on Paraffin Sections: 8-25 μg/mL. Positive Control: Human breast tissue.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Sterile PBS

Storage: -20 °C

Storage Comment: Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: parp1

Background: PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like Caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP helps cells to maintain their viability cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis.

UniProt: P09874

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location