PRSS1 antibody (HRP)

Catalog No. ABIN5541698

This Rabbit Polyclonal antibody specifically detects PRSS1 in WB, EIA, IHC (p) and IHC (fro). It exhibits reactivity toward Cow.

Quick Overview for PRSS1 antibody (HRP) (ABIN5541698)

Target: PRSS1 (Protease, serine, 1 (Trypsin 1) (PRSS1))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRSS1 antibody is conjugated to HRP

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PRSS1 Antibody (HRP)

Specificity: This antibody detects bovine trypsin. Cross reactivity from other sources is unknown. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified trypsin [bovine pancreas].

Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer

Immunogen: Trypsin from bovine pancreas

Application Details

Application Notes: ELISA : 1/14,000. Western blot: 1/1,000-1/5,000. Immunohistochemistry : Paraffin (FFPE) or Frozen sections 1/100 suggested to begin.

Restrictions: For Research Use only

Handling

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for PRSS1

Target: PRSS1 (Protease, serine, 1 (Trypsin 1) (PRSS1))

Alternative Name: trypsin-1,prss1

Background: Trypsin is a 24 kDa enzyme of the serine proteinase family. It is produced in the pancreas as an inactive precursor, trypsinogen, but the active enzyme is located in the gastrointestinal tract where it degrades proteins to large peptides. Trypsin prefers lysine and arginine residues. One of the substrates for trypsin is chymotrypsinogen which is cleaved to produce active chymotrypsin. High levels of immunoreactive trypsin in the bloodstream can indicate pancreatic malfunction and this indicator is used to screen for and diagnose Cystic Fibrosis.

UniProt: P00760