ADH1 antibody (Biotin)

Catalog No. ABIN5541710

This Rabbit Polyclonal antibody specifically detects ADH1 in WB, IP and EIA. It exhibits reactivity toward Saccharomyces cerevisiae.

Quick Overview for ADH1 antibody (Biotin) (ABIN5541710)

Target: ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADH1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-ADH1 Antibody (Biotin)

Specificity: This antibody detects yeast alcohol dehydrogenase. Cross reactivity against alcohol dehydrogenase from other sources may occur but have not been specifically determined. Immunoelectrophoresis gives a single precipitin arc against anti-Biotin, anti-rabbit serum as well as purified and partially purified yeast alcohol dehydrogenase.

Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer

Immunogen: Alcohol dehydrogenase from yeast

Application Details

Application Notes: Western blot : 1/500-1/5,000. Immunoprecipitation : 1/100. ELISA : 1/5,000-1/20,000. Antibody has been assayed against 1.0 μg of Alcohol dehydrogenase in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested.

Restrictions: For Research Use only

Handling

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for ADH1

Target: ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))

Alternative Name: alcohol dehydrogenase 1

UniProt: P00330