ACTH antibody (AA 1-24)

Catalog No. ABIN5542231

This anti-ACTH antibody is a Mouse Monoclonal antibody detecting ACTH in IHC (p) and EIA. Suitable for Human and Rat.

Quick Overview for ACTH antibody (AA 1-24) (ABIN5542231)

Target: ACTH (Adrenocorticotropic hormone (ACTH))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ACTH antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 57 (BGN-1388-66)

Product Details anti-ACTH Antibody

Binding Specificity: AA 1-24

Sequence: SYSMEHFRWG KPVGKKRRPV KVYP

Specificity: This antibody recognizes 1-24 ACTH (Synacthen) which, as a synthetic analogue of naturally-occurring Adrenocorticotropic Hormone, can be used to measure adrenal reserve and for the diagnosis of adrenal insufficiency by acute adrenocortical stimulation. It does not recognize 17-39 ACTH (CLIP). Species: Human. Cross-reacts with Rat N-terminal ACTH.

Purification: Affinity Chromatography on Protein A

Immunogen: Synthetic peptide corresponding to the amino acids 1-24 of the Human ACTH conjugated to Keyhole Limpet Haemocyanin.

Isotype: IgG1

Application Details

Application Notes: ELISA. Immunohistochemistry on Formalin Fixed Paraffin Embedded Sections: 1/100-1/200 (Requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.2 Preservatives: 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for ACTH

Target: ACTH (Adrenocorticotropic hormone (ACTH))

Target Type: Hormone

Background: ACTH, released from the anterior pituitary gland in response to corticotropin-releasing hormone from the hypothalamus, acts on the adrenal cortex to stimulate the production of corticosteroids such as cortisol, involved in the response to stress. Studies have shown that the administration of 1-24 ACTH increases blood pressure, believed to be attributed to an increase in ACTH-stimulated cortisol secretion, in association with increased cardiac output.

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity