GLI1 antibody (AA 284-449)

Catalog No. ABIN5542320

The Mouse Monoclonal anti-GLI1 antibody has been validated for ELISA, IHC, FACS and ICC. It is suitable to detect GLI1 in samples from Human.

Quick Overview for GLI1 antibody (AA 284-449) (ABIN5542320)

Target: GLI1 (Zinc Finger Protein GLI1 (GLI1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GLI1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 1B9F8

Product Details anti-GLI1 Antibody

Binding Specificity: AA 284-449

Purpose: GLI1 Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human GLI1 (AA: 284-449) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Target Details for GLI1

Target: GLI1 (Zinc Finger Protein GLI1 (GLI1))

Alternative Name: GLI1

Background: This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 118 kDa

Gene ID: 2735

UniProt: P08151

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis