SYN1 antibody (AA 362-511)

Catalog No. ABIN5542390

This anti-SYN1 antibody is a Mouse Monoclonal antibody detecting SYN1 in WB, ELISA, IHC, ICC and FACS. Suitable for Human, Rat, Mouse and Monkey. Independently validated for use in Multiplex Immunohistochemistry.

Quick Overview for SYN1 antibody (AA 362-511) (ABIN5542390)

Target: SYN1 (Synapsin I (SYN1))

Reactivity: Human, Rat, Mouse, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This SYN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 7H10G6

Product Details anti-SYN1 Antibody

Binding Specificity: AA 362-511

Purpose: SYN1 Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human SYN1 (AA: 362-511) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Independent Validation (mIHC)

Validation #104335 (Multiplex Immunohistochemistry)

by: Akoya Biosciences

No.: #104335

Date: 04/20/2021

Antigen: SYN1

Lot Number: 151229

Method validated: Multiplex Immunohistochemistry

Positive Control:

FFPE normal human cortex

Negative Control:

Unlabeled control (FFPE normal human cortex)

Notes:

Passed. The anti-SYN1 antibody ABIN5542390 produces punctate labeling in synapse-rich regions of the human cortex. This pattern is consistent with expected SYN1 localization in synaptic contacts.

Validation Images

FFPE normal human cortex tissue section labeled with anti-SYN1 antibody ABIN5542390 (blue; bound to fluorophore AF488) after EDTA antigen retrieval. MAP2 and SLC32A1 were labeled with anti-MAP2 antibody ABIN125739 (red; bound to fluorophore ATTO 500) and anti-SLC32A1 antibody ABIN2855225 (green; bound to fluorophore AF488).

FFPE normal human cortex tissue section labeled with anti-SYN1 antibody ABIN5542390 (red; bound to fluorophore AF488) after EDTA antigen retrieval. MAP2 and DLG4 were labeled with anti-MAP2 antibody ABIN125739 (cyan; bound to fluorophore ATTO 550) and anti-DLG4 antibody ABIN361694 (green; bound to fluorophore ATTO 550).

Full Methods

Primary Antibody: ABIN5542390

Full Protocol:

• Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
• Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
• Conjugation of the anti-SYN1 antibody ABIN5542390 to an oligo barcode used to bind oligo-conjugated fluorophore AF488.

Experimental Notes:

• No signal was detected in unlabeled specimens.

• Specific staining of Cortactin was also observed with human FFPE cortical tissue sections with both citrate antigen retrieval and EDTA antigen retrieval.

• Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Target Details for SYN1

Target: SYN1 (Synapsin I (SYN1))

Alternative Name: SYN1

Background: This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein encoded serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations in this gene may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.

Molecular Weight: 74.1 kDa

Gene ID: 6853

UniProt: P17600