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Ezrin antibody (AA 292-464)

Name: Ezrin antibody (AA 292-464)
Brand: antibodies-online
SKU: ABIN5542456
Price: 533.5 USD
Availability: InStock

This anti-Ezrin antibody is a Mouse Monoclonal antibody detecting Ezrin in WB, ELISA, ICC and FACS. Suitable for Human, Mouse and Monkey. This Primary Antibody has been cited in 1 publication.

Western blot analysis using EZR mAb against human EZR (AA: 292-464) recombinant protein. (Ezrin antibody (AA 292-464))

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng), (Ezrin antibody (AA 292-464))

ELISA

Western blot analysis using EZR mAb against HEK293 (1) and EZR (AA: 292-464)-hIgGFc transfected HEK293 (2) cell lysate. (Ezrin antibody (AA 292-464))

Western blot analysis using EZR mouse mAb against MCF-7 (1), Hela (2), A431 (3), Hek293 (4), SK-N-SH (5), Jurkat (6), HepG2 (7), NIH/3T3 (8), and Cos7 (9) cell lysate. (Ezrin antibody (AA 292-464))

Flow cytometric analysis of MCF-7 cells using EZR mouse mAb (green) and negative control (red). (Ezrin antibody (AA 292-464))

FACS

Flow cytometric analysis of Hela cells using EZR mouse mAb (green) and negative control (red). (Ezrin antibody (AA 292-464))

FACS

Immunofluorescence analysis of A549 cells using EZR mouse mAb (green). (Ezrin antibody (AA 292-464))

7 images

(1 reference)

Catalog No. ABIN5542456

Quick Overview for Ezrin antibody (AA 292-464) (ABIN5542456)

Target

See all Ezrin (EZR) Antibodies

Ezrin (EZR)

Reactivity

Human, Mouse, Monkey

Host

Mouse

Clonality

Monoclonal

Conjugate

This Ezrin antibody is un-conjugated

Application

Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone

6F1A9

Binding Specificity
28

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AA 292-464

Purpose

EZR Antibody

Purification

Purified antibody

Immunogen

Purified recombinant fragment of human EZR (AA: 292-464) expressed in E. Coli.

Isotype

IgG1
anti-Ezrin (EZR) (AA 1-295) antibody
EZR Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Ezrin (EZR) (pTyr478) antibody
EZR Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Ezrin (EZR) (AA 1-586) antibody
EZR Reactivity: Human WB, IP Host: Rabbit Polyclonal unconjugated

anti-Ezrin (EZR) (pTyr353) antibody
EZR Reactivity: Human, Mouse, Rat WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Ezrin (EZR) antibody
EZR Reactivity: Human WB, IHC, StM Host: Mouse Monoclonal CPTC-Ezrin-1 unconjugated

anti-Ezrin (EZR) (AA 1-586) antibody
EZR Reactivity: Human WB, ELISA, IHC (p), PLA Host: Mouse Monoclonal 1E11 unconjugated

anti-Ezrin (EZR) (AA 350-470) antibody
EZR Reactivity: Human WB, IP, IF Host: Rabbit Polyclonal unconjugated

anti-Ezrin (EZR) (AA 1-251) antibody
EZR Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Ezrin (EZR) (AA 455-586) antibody
EZR Reactivity: Human, Rat WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Ezrin (EZR) (C-Term) antibody
EZR Reactivity: Human, Mouse, Rat WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

ELISA: 1/10000
FCM: 1/200 - 1/400
ICC: 1/100 - 1/400

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Popov, Brazhe, Fedotova, Tiaglik, Bychkov, Morozova, Brazhe, Aronov, Lyukmanova, Lazareva, Li, Ponimaskin, Verkhratsky, Semyanov: "A high-fat diet changes astrocytic metabolism to promote synaptic plasticity and behavior." in: Acta physiologica (Oxford, England), pp. e13847, (2022) (PubMed).
Target

Ezrin (EZR)

Alternative Name

EZR

Background

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.

Molecular Weight

69.4 kDa

UniProt

P15311

Pathways

Maintenance of Protein Location