EN1 antibody (N-Term)

Catalog No. ABIN5552216

This Rabbit Polyclonal antibody specifically detects EN1 in WB, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for EN1 antibody (N-Term) (ABIN5552216)

Target: EN1 (Engrailed Homeobox 1 (EN1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-EN1 Antibody

Binding Specificity: N-Term

Specificity: This antibody detects Engrailed 1 at N-term.

Purification: Protein A column, followed by peptide affinity purification.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human EN1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA 1: 1,000. Western blot 1: 50 - 1: 100. Immunohistochemistry 1: 10 - 1: 50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for EN1

Target: EN1 (Engrailed Homeobox 1 (EN1))

Alternative Name: Engrailed-1 / EN1

Background: Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.Synonyms: Homeobox protein engrailed-1

Molecular Weight: 40101 Da

Gene ID: 2019, 9606

UniProt: Q05925

Pathways: Dopaminergic Neurogenesis