USP11 antibody (C-Term)

Catalog No. ABIN374330

The Goat Polyclonal anti-USP11 antibody is suitable to detect USP11 in samples from Human. It has been validated for WB.

Quick Overview for USP11 antibody (C-Term) (ABIN374330)

Target: USP11 (Ubiquitin Specific Peptidase 11 (USP11))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This USP11 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-USP11 Antibody

Binding Specificity: C-Term

Sequence: CSSPPSSEFM DVN

Specificity: This antibody detects USP11 at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_004642.2. Genename: USP11

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 8000. Western blot: 1 μg/mL. Approx 120-130 kDa band observed in Jurkat and Human Testislysates (despite a predicted MW of 110 according to NP_004642 this protein has previouslybeen reported to run significantly higher (Ideguchi et al, see ref below)).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for USP11

Target: USP11 (Ubiquitin Specific Peptidase 11 (USP11))

Alternative Name: USP11

Background: Synonyms: Deubiquitinating enzyme 11, UHX1, Ubiquitin carboxyl-terminal hydrolase 11, Ubiquitin thioesterase 11, Ubiquitin-specific-processing protease 11

Gene ID: 8237

NCBI Accession: NP_004642

UniProt: P51784