GRIN3B antibody (C-Term)

Catalog No. ABIN498089

The Goat Polyclonal anti-GRIN3B antibody is suitable to detect GRIN3B in samples from Human. It has been validated for WB and EIA.

Quick Overview for GRIN3B antibody (C-Term) (ABIN498089)

Target: GRIN3B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 3B (GRIN3B))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GRIN3B antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-GRIN3B Antibody

Binding Specificity: C-Term

Sequence: C-EAPPHSGRPG SQ

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_619635.1. Genename: GRIN3B

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/8000. Western blot: 0.5-1.5 μg/mL. Detects a band of Approx 110 kDa in Human Brain (Cerebellum and Cerebral Cortex) lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Species Reactivity: Tested: Human. Add. Information: Calculated Molecular Weight: 110 kDa according to NP_619635.1

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GRIN3B

Target: GRIN3B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 3B (GRIN3B))

Alternative Name: GRIN3B

Background: NR3B is a NMDA receptor subtype of glutamate-gated ion channels with reduced single-channel conductance, low calcium permeability and low voltage-dependent sensitivity to magnesium. NR3B is found in a complex containing GRIN1 and GRIN2A at the cell membrane. NR3B requires the presence of GRIN1 to be targeted at the cell membrane. NR3B is expressed in the hippocampus, corpus callosum, as well as the facial and trigeminal nuclei of the brainstem and the ventral horn of the spinal cord.Synonyms: Glutamate [NMDA] receptor subunit 3B, NMDAR3B, NR3B

Gene ID: 116444

NCBI Accession: NP_619635

UniProt: O60391