UBE2F antibody (N-Term)

Catalog No. ABIN615974

The Goat Polyclonal anti-UBE2F antibody is suitable to detect UBE2F in samples from Human, Mouse, Rat, Cow, Dog and Pig. It has been validated for WB and EIA.

Quick Overview for UBE2F antibody (N-Term) (ABIN615974)

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This UBE2F antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-UBE2F Antibody

Binding Specificity: N-Term

Sequence: SKLKRDDGLK GSRT-C

Specificity: Recogfnizes NEDD8 conjugating enzyme (N Terminus).

Cross-Reactivity (Details): Species reactivity (expected):Human, Mouse, Canine (Dog), Pig, Cow.
Species reactivity (tested):Rat.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the N Terminus of the protein sequence according to NP_542409.1. Genename: UBE2F

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for UBE2F

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Alternative Name: NCE2

Background: Ubiquitin is covalently attached to target proteins by a multienzymatic system consisting of E1 (ubiquitin-activating), E2 (ubiquitin-conjugating), and E3 (ubiquitin-ligating) enzymes. NEDD8, a ubiquitin-like protein, is conjugated to proteins in a manner analogous to ubiquitinylation. beta-amyloid precursor protein-binding protein-1 (APPBP1) can bind to NEDD8 in rabbit reticulocyte lysates. However, since APPBP1 shows similarity to only the N-terminal domain of an E1 enzyme, it must interact with a protein showing similarity to the C-terminal region of E1s. By searching sequence databases, a cDNAs encoding UBA3 was identified as the human homolog of yeast Uba3. The predicted 442-amino acid UBA3 protein shares 43 % sequence identity with yeast Uba3. In vitro, UBA3 formed a complex with APPBP1 and a thioester linkage with NEDD8. APPBP1/UBA3 complex may function as an E1-like enzyme for the activation of NEDD8.Synonyms: NEDD8-conjugating enzyme 2, UBE2F, Ubiquitin-conjugating enzyme E2 F

Gene ID: 140739

NCBI Accession: NP_542409

UniProt: Q969M7