Recombinant POLR2F antibody

Catalog No. ABIN5563983

The Mouse Monoclonal anti-POLR2F antibody has been validated for WB, IF, ChIP and ChIP-seq. It is suitable to detect POLR2F in samples from Human.

Quick Overview for Recombinant POLR2F antibody (ABIN5563983)

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This POLR2F antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Product Details anti-POLR2F Antibody

Brand: AbFlex®

Characteristics: AbFlex™ RNA Pol II antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Purification: Protein A

Isotype: IgG2a

Application Details

Application Notes: ChIP-Seq: 4 μg per ChIP Western Blot: 0.1 - 1 μg/mL Immunofluorescence (IF): 2 μg/mL.

Comment:

AbFlex® Recombinant Antibodies defined antibodies for highly specific, reproducible performance.
AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. The unique advantages of the AbFlex® antibody are its flexible labeling and purification options. Each AbFlex® antibody contains a Sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner using our Sortag-IT Labeling Kits. Every antibody also contains a 6xHis tag, which can be used with nickel-based purification systems, and an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.

AbFlex® antibodies are specifically labeled at the end of the constant region of the heavy chain to avoid interference with antigen recognition and functionality. This is important as it ensures the labeling process maintains the integrity of the antibody so signal is not diminished as a result of non-functional antibodies. In contrast, commonly used chemical labeling methods add labels to the antibody in a random fashion. The randomness of this process has a high potential to block the antigen-binding site and render the antibody ineffective. Chemical labeling can also deposit labels on the Fc region of the antibody which has the potential to obstruct interactions with protein A.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 35 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for POLR2F

Target: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Alternative Name: RNA Pol II

Molecular Weight: 217 kDa

Pathways: Regulatory RNA Pathways