Recombinant Angiopoietin 2 antibody

Catalog No. ABIN5564224

This anti-Angiopoietin 2 antibody is a Mouse Monoclonal antibody detecting Angiopoietin 2 in ELISA and Func. Suitable for Human and Mouse.

Quick Overview for Recombinant Angiopoietin 2 antibody (ABIN5564224)

Target: Angiopoietin 2 (ANGPT2)

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Angiopoietin 2 antibody is un-conjugated

Application: ELISA, Functional Studies (Func)

Clone: Angy-2-1

Product Details anti-Angiopoietin 2 Antibody

Specificity: Recognizes human and mouse angiopoietin-2.

Cross-Reactivity: Human, Mouse (Murine)

Cross-Reactivity (Details): Does not detect human angiopoietin-1.

Purity: >95 % (SDS-PAGE)

Immunogen: Recombinant human angiopoietin-2.

Isotype: IgG2b

Application Details

Comment:

anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) (preservative free) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the mouse lgG2b Fc domain.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 10 % glycerol.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

Target Details for Angiopoietin 2

Target: Angiopoietin 2 (ANGPT2)

Alternative Name: Angiopoietin-2

Background: Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are closely related secreted ligands which bind with similar affinity to Tie-2. Tie-2 and angiopoietins have been shown to play critical roles in embryogenic angiogenesis and in maintaining the integrity of the adult vasculature. Ang-1 activates Tie-2 signaling on endothelial cells to promote chemotaxis, cell survival, cell sprouting, vessel growth and stabilization. Ang-2 has been identified as a secreted protein ligand of Tie-2 and has alternatively been reported to be an antagonist for Ang-1 induced Tie-2 signaling as well as an agonist for Tie-2 signaling, depending on the cell context. anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) (preservative free) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

UniProt: O15123

Pathways: RTK Signaling