Caspase 2 antibody (Subunit p19)

Catalog No. ABIN5564278

This anti-Caspase 2 antibody is a Rat Monoclonal antibody detecting Caspase 2 in WB, ELISA, IHC, ICC, IP and FACS. Suitable for Human, Mouse, Rat, Dog and Monkey.

Quick Overview for Caspase 2 antibody (Subunit p19) (ABIN5564278)

Target: Caspase 2 (CASP2) (Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2))

Reactivity: Human, Mouse, Rat, Dog, Monkey

Host: Rat

Clonality: Monoclonal

Conjugate: This Caspase 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FACS)

Clone: 11B4

Product Details anti-Caspase 2 Antibody

Binding Specificity: Subunit p19

Specificity: Recognizes an epitope in the p19 subunit of human, mouse, rat, monkey and dog caspase-2. Detects bands of ~51 kDa (procaspase-2), ~32 kDa and ~18 kDa (cleavage products) by Western blot.

Cross-Reactivity: Dog (Canine), Human, Monkey, Mouse (Murine), Rat (Rattus)

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.1EU/μg purified protein (LAL test, Lonza).

Immunogen: Recombinant human caspase-2 (p19 subunit).

Isotype: IgG2a

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

Target Details for Caspase 2

Target: Caspase 2 (CASP2) (Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2))

Alternative Name: Caspase-2

Background: Caspase-2 is a Class I caspase with a long prodomain necessary for nuclear localization. Upon activation of the apoptotic pathway, the procaspase is cleaved into smaller fragments. Caspase-2 is the nuclear apoptotic respondent to cellular genotoxic stress or mitotic catastrophe and is involved in the activation cascade of caspases responsible for apoptosis execution. Activation occurs upon recruitment to a complex containing a p53-induced death domain protein, PIDD. This suggests caspase-2 can be a nuclear initiator caspase with a requirement for caspase-9 and caspase-3 activation in downstream apoptotic events. In apoptotic pathways resulting from UV-induced DNA damage, processing of caspase-2 occurs downstream of mitochondrial dysfunction and of caspase-9 and caspase-3 activation, extending a possible role for caspase-2 as a parallel effector caspase.

UniProt: P42575

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Neurotrophin Signaling Pathway