Recombinant Myosin 9 antibody (Atto 488)

Catalog No. ABIN5564289

The Human Monoclonal anti-Myosin 9 antibody has been validated for ICC. It is suitable to detect Myosin 9 in samples from Human, Mouse, Rat and Drosophila melanogaster.

Quick Overview for Recombinant Myosin 9 antibody (Atto 488) (ABIN5564289)

Target: Myosin 9 (MYH9)

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat, Drosophila melanogaster

Host: Human

Clonality: Monoclonal

Conjugate: This Myosin 9 antibody is conjugated to Atto 488

Application: Immunocytochemistry (ICC)

Clone: SF9

Product Details anti-Myosin 9 Antibody (Atto 488)

Specificity: Recognizes human, mouse, rat and drosophila myosin IIA (heavy chain).

Cross-Reactivity: Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus)

Purity: >95 % (SDS-PAGE)

Immunogen: Full length myosin IIA from rat liver.

Isotype: IgG2

Application Details

Comment:

anti-Myosin IIA (non-muscle), monoclonal antibody (recombinant) (SF9) (ATTO 488) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain and labeled with ATTO 488.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: +4°C
Keep conjugated formats at +4°C.

Target Details for Myosin 9

Target: Myosin 9 (MYH9)

Alternative Name: Myosin IIA

Background: Anti-Myosin IIA (non-muscle), monoclonal antibody (recombinant) (SF9) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

UniProt: P35579

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Integrin Complex