ADA antibody (HRP)

Catalog No. ABIN5596683

Supplier Product No.: 200-403-140-0100

This Rabbit Polyclonal antibody specifically detects ADA in WB, ELISA and IP. It exhibits reactivity toward Cow.

Quick Overview for ADA antibody (HRP) (ABIN5596683)

Target: ADA (Adenosine Deaminase (ADA))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADA antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-ADA Antibody (HRP)

Supplier Product No.: 200-403-140-0100

Supplier: Rockland

Purpose: Adenosine Deaminase Antibody Peroxidase Conjugated

Characteristics: Synonyms: rabbit anti-Adenosine Deaminase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Adenosine Deaminase Antibody, Adenosine deaminase, Adenosine aminohydrolase

Purification: Anti-Adenosine deaminase Peroxidase antibody has been tested by ELISA and western blot and is assayed against 1.0 μg of Adenosine Deaminase [Calf Spleen] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.

Immunogen:

Immunogen: Adenosine Deaminase [Calf Spleen] ADA

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Adenosine deaminase has been assayed against 1.0 μg of Adenosine Deaminase [Calf Spleen] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for ADA

Target: ADA (Adenosine Deaminase (ADA))

Alternative Name: ADA

Background: Background: Adenosine deaminase (adenosine aminhydrolase, or ADA) is an enzyme involved in purine metabolism. It is needed for the breakdown of adenosine from food and for the turnover of nucleic acids in tissues. ADA irreversibly deaminates adenosine, converting it to the related nucleoside inosine by the substitution of the amino group for a hydroxyl group. ADA irreversibly deaminates adenosine, converting it to the related nucleoside inosine by the substitution of the amino group for a hydroxyl group. Inosine can then be deribosylated by another enzyme called purine nucleoside phosphorylase (PNP), converting it to hypoxanthine.

Gene ID: 280712

NCBI Accession: NP_776312

UniProt: P56658

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Ribonucleoside Biosynthetic Process