ALD2 antibody (HRP)

Catalog No. ABIN5596684

Supplier Product No.: 200-4344-0100

This anti- antibody is a Rabbit Polyclonal antibody detecting in ELISA, IP and WB. Suitable for Saccharomyces cerevisiae.

Quick Overview for ALD2 antibody (HRP) (ABIN5596684)

Target: ALD2

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunoprecipitation (IP), Western Blotting (WB)

Product Details

Supplier Product No.: 200-4344-0100

Supplier: Rockland

Purpose: Aldehyde Dehydrogenase Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Aldehyde Dehydrogenase [Yeast].

Characteristics: Synonyms: rabbit anti-Aldehyde Dehydrogenase Antibody Peroxidase Conjugation, HRP conjugated anti-Aldehyde Dehydrogenase Antibody, Aldehyde dehydrogenase [NAD(P)+] 1, ALD2, ALD5, ALD

Purification: Aldehyde dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Aldehyde Dehydrogenase [Yeast]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Aldehyde Dehydrogenase Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is assayed against 1.0 μg of Aldehyde Dehydrogenase [Yeast] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: ALD2

Background: Background: Aldehyde dehydrogenase are a group of enzymes that catalyze the oxidation (dehydrogenation) of aldehydes. Aldehyde dehydrogenase is a polymorphic enzyme responsible for the oxidation of aldehydes to carboxylic acids, which leave the liver and are metabolized by the body's muscle and heart. ALDH2 also plays a crucial role in maintaining low blood levels of acetaldehyde during alcohol oxidation.

Gene ID: 855206

NCBI Accession: NP_013893

UniProt: P47771