Choline Oxidase antibody (HRP)

Catalog No. ABIN5596705

Supplier Product No.: 200-103-094-0100

This anti-Choline Oxidase antibody is a Goat Polyclonal antibody detecting Choline Oxidase in WB, ELISA and IP. Suitable for Alcaligenes.

Quick Overview for Choline Oxidase antibody (HRP) (ABIN5596705)

Target: Choline Oxidase

Reactivity: Alcaligenes

Host: Goat

Clonality: Polyclonal

Conjugate: This Choline Oxidase antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-Choline Oxidase Antibody (HRP)

Supplier Product No.: 200-103-094-0100

Supplier: Rockland

Purpose: Choline Oxidase Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Choline Oxidase [Alcaligenes species].

Characteristics: Synonyms: goat anti-Choline Oxidase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Choline Oxidase Antibody, codA antibody

Purification: Anti-Choline oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Choline Oxidase [Alcaligenes species]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Choline oxidase has been tested by ELISA and western blot and is assayed against 1.0 μg of Choline Oxidase [Alcaligenes species] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:1,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:1,000 - 1:2,500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Choline Oxidase

Target: Choline Oxidase

Background: Background: Choline oxidase is an enzyme that catalyzes the chemical reaction (choline + O2 <=> betaine aldehyde + H2O2). Thus, the two substrates of this enzyme are choline and O2, whereas its two products are betaine aldehyde and H2O2. Choline oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. This enzyme participates in glycine, serine, and threonine metabolism.

Gene ID: 116367

UniProt: P16101