HDAC5 antibody (AA 200-250)

Catalog No. ABIN5596726

Supplier Product No.: 600-401-j70

This anti-HDAC5 antibody is a Rabbit Polyclonal antibody detecting HDAC5 in WB, IHC and ELISA. Suitable for Human.

Quick Overview for HDAC5 antibody (AA 200-250) (ABIN5596726)

Target: HDAC5 (Histone Deacetylase 5 (HDAC5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-HDAC5 Antibody

Binding Specificity: AA 200-250

Supplier Product No.: 600-401-j70

Supplier: Rockland

Purpose: HDAC5 (internal) Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with human, mouse, and primate based on 100 % sequence homology.

Characteristics: Synonyms: rabbit anti-HDAC-5 antibody, HDAC5, HDAC 5, HD 5 antibody, histone deacetylase 5 antibody, Histone deacetylase-5, HD5 antibody, NY-CO-9

Purification: Anti-HDAC5 was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-HDAC5 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region surrounding 200-250aa of human HDAC5 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 5 μg/mL

Application Note: Anti-HDAC5 antibody has been tested by ELISA, Immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~122 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1: 10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for HDAC5

Target: HDAC5 (Histone Deacetylase 5 (HDAC5))

Alternative Name: HDAC5

Background: Background: HDAC5 is a ubiquitous protein responsible for the deacetylation of lysine residues on the N-terminal part of the core histones. A tag for epigenetic repression, Anti-HDAC5 is an enzyme involved in transcriptional regulation, cell cycle progression and developmental events. HDAC5 is a class II histone deacetylase as defined by its similarities to other members of this class. HDAC5 is a major player in muscle differentiation, acting by repressing transcription of myocyte enhancer MEF2C. During differentiation it moves from the nucleus into the cytoplasm, allowing for myocyte enhancer factor expression. Anti-HDAC5 antibody is ideal for researchers interested in Stem Cell , Cardiovascular , or Epigenetics and Nuclear Signaling research.

UniProt: Q9UQL6

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Monocarboxylic Acid Catabolic Process