HDAC8 antibody (C-Term)

Catalog No. ABIN5596727

Supplier Product No.: 600-401-j71

This Rabbit Polyclonal antibody specifically detects HDAC8 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for HDAC8 antibody (C-Term) (ABIN5596727)

Target: HDAC8 (Histone Deacetylase 8 (HDAC8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HDAC8 Antibody

Binding Specificity: C-Term

Supplier Product No.: 600-401-j71

Supplier: Rockland

Purpose: HDAC8 (C-terminus) Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with rat, mouse, human, and bovine based on 100 % sequence homology.

Characteristics: Synonyms: rabbit anti-HDAC-8 antibody, HDAC8, HDAC 8, HD-8, HD 8, Histone deacetylase 8, Histone deacetylase-8, HD8, HDACL1, CDA07

Purification: Anti-HDAC8 was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-HDAC8 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the C-terminus of human HDAC8 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-HDAC8 antibody has been tested by ELISA and western blot and is useful in Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~41 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1: 10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for HDAC8

Target: HDAC8 (Histone Deacetylase 8 (HDAC8))

Alternative Name: HDAC8

Background: Background: HDAC8 is located in the nucleus and cytoplasm, expressed weakly in most tissues with higher expression in the heart, brain, kidney, pancreas, liver, lunch, placenta, prostate, and kidney. It is responsible for the deacetylation of lysine residues on the N-terminal region of the core histones (H2A, H2B, H3 and H4). The result of deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Anti-HDAC8 therefore is ideal for investigators interested in Stem Cells , Cardiovascular , or Epigenetics and Nuclear Signaling research.

UniProt: Q9BY41

Pathways: Cellular Glucan Metabolic Process