HDAC9 antibody (Internal Region)

Catalog No. ABIN5596730

Supplier Product No.: 600-401-j74

This Rabbit Polyclonal antibody specifically detects HDAC9 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for HDAC9 antibody (Internal Region) (ABIN5596730)

Target: HDAC9 (Histone Deacetylase 9 (HDAC9))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-HDAC9 Antibody

Binding Specificity: Internal Region

Supplier Product No.: 600-401-j74

Supplier: Rockland

Purpose: HDAC9 (internal) Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with human based on 100 % sequence homology.

Characteristics: Synonyms: rabbit anti-HDAC-9 antibody, HDAC9, HDAC 9, HD-9, HD 9, Histone deacetylase 9, Histone deacetylase-9, HD9, Histone deacetylase 7B, HD7, HD7b, Histone deacetylase-related protein, MEF2-interacting transcription repressor MITR, HDAC7, HDAC7B, HDRP, MITR

Purification: Anti-HDAC9 was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-HDAC9 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region of human HDAC9 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 5 μg/mL

Application Note: Anti-HDAC9 antibody is tested by Immunohistochemistry and useful for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~111 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1: 10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for HDAC9

Target: HDAC9 (Histone Deacetylase 9 (HDAC9))

Alternative Name: HDAC9

Background: Background: HDAC9 is located in the nucleus, expressed most highly in brain, heart, muscle, and testis. It is responsible for the deacetylation of lysine residues on the N-terminal region of the core histones (H2A, H2B, H3 and H4). The result of deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC9 represses MEF2-dependent transcription by recruiting HDAC1 and 3. It appears to inhibit skeletal myogenesis and be a factor in heart development. By repressing JUN transcription via HDAC1 and inhibiting JUN phosphorylation by MAPK10, HDAC9 protects neurons from apoptosis. Anti-HDAC9 therefore is ideal for investigators interested in Cardiovascular or Epigenetics and Nuclear Signaling research.

UniProt: Q9UKV0

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development