Ovalbumin antibody (FITC)

Catalog No. ABIN5596746

Supplier Product No.: 200-4233-0100

Product Details anti-Ovalbumin Antibody (FITC)

Target: Ovalbumin (OVA)

Reactivity: Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ovalbumin antibody is conjugated to FITC

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Supplier Product No.: 200-4233-0100

Supplier: Rockland

Purpose: Ovalbumin Antibody Fluorescein Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White].

Characteristics: Synonyms: rabbit anti-Ovalbumin Antibody FITC Conjugation, fluorescein conjugated rabbit anti-Ovalbumin antibody, Egg albumin antibody, Plakalbumin, Allergen Gal d II, Allergen=Gal d 2, Hen Egg White

Purification: Ovalbumin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Ovalbumin [Hen Egg White]

Immunogen Type: Native Protein

Isotype: IgG

Labeling Ratio: 3.57

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Application Note: Anti-Ovalbumin fluorescein conjugated antibody has been tested by ELISA, dot blot, and western blot and is suitable for fluorescent western blot, immunofluorescence microscopy, and flow cytometry or FACS analysis as well as other antibody based fluorescent assays. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: User Optimized

Immunoprecipitation Dilution: 1:100

IF Microscopy Dilution: 1:500 - 1:2,500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Ovalbumin

Target: Ovalbumin (OVA)

Alternative Name: SERPINB14 (OVA Products)

Background: Background: Anti-Ovalbumin Antibody detects Ovalbumin. Ovalbumin is the main protein found in egg white, making up 60-65 % of the total protein. It is a non-inhibitory serpin. It is the storage protein of egg white. Anti-Ovalbumin Antibody is ideal for investigators involved in Cell Signaling, Immunology and Signal Transduction research.

Gene ID: 396058

UniProt: P01012