STIM1 antibody (N-Term)

Catalog No. ABIN5596754

Supplier Product No.: 600-601-j49

This Sheep Polyclonal antibody specifically detects STIM1 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for STIM1 antibody (N-Term) (ABIN5596754)

Target: STIM1 (Stromal Interaction Molecule 1 (STIM1))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This STIM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-STIM1 Antibody

Binding Specificity: N-Term

Supplier Product No.: 600-601-j49

Supplier: Rockland

Purpose: STIM1 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with rat, human, and mouse based on 100 % sequence homology.

Characteristics: Synonyms: sheep anti-Stim1 Antibody, STIM-1, STIM 1, Stromal interaction molecule 1, GOK

Purification: Anti-Stim I was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Stim 1 affinity purified antibody was prepared from whole sheep serum produced by repeated immunizations with a synthetic peptide near the N-terminus of human Stim 1.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 5 μg/mL

Application Note: Anti-Stim I antibody is tested for ELISA and immunohistochemistry and suitable for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~77 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1: 10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.12 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol with 1 mg/mL Bovine Serum Albumin (BSA)

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for STIM1

Target: STIM1 (Stromal Interaction Molecule 1 (STIM1))

Alternative Name: STIM1

Background: Background: Stim I antibody helps regulate calcium influx after the depletion of intracellular calcium stores. It functions as a calcium sensor in the endoplasmic reticulum via its EF-hand domain. After depletion of Ca+2, it is translocated from the endoplasmic reticulum to the plasma membrane so the calcium release-activated channel subunit is activated. Anti-Stim I antibody is ideal for investigators interested in Metabolism and Signal Transduction research.

Gene ID: 6786

NCBI Accession: NP_003147

UniProt: Q13586

Pathways: TCR Signaling, BCR Signaling